[vtkusers] green and red not mixing to make yellow, in raycastmapper???

[Lisa Avila]

Hello Dan,

The volume mappers in VTK cannot render overlapping volume data. Unless 
your two image stacks have non-overlapping bounding boxes, they cannot be 
rendered in the same scene.

Lisa


At 06:47 AM 10/15/2003, Dr. Daniel James White PhD wrote:
>Hi,
>
>I've been following the discussion on the VTK list about ray casting and 
>blending,
>I am a novice so please bear with me....
>
>I think I am experiencing this problem with VTK,
>attached is a screen capture from MayaVi (VTK python frontend)
>where I have loaded 2 data sets of a 3D volume of the same living cell 
>taken by confocal microscopy.
>These are 2 different fluorescence channels, which I represent as green 
>and red.
>(They are really from 2 grayscale tiff stacks)
>Using the VTK raycastmapper with the compositefunction I expected to see 
>yellow where there is signal in both the red and green channels (we call 
>this colocalisation). same happens with the MIPfunction
>
>But as you can see in the attached jpg I either get red or green only.
>In the blobs (matrix vesicles) around the edge of the cell there should be 
>some yellow visible.
>You can see there are some blobs with green outer and red inner.....
>
>Is this the same problem that you guys are talking about?
>
>cheers
>
>Dan
>
>
>
>
>
>Dr. Daniel James White BSc. (Hons.) PhD
>Cell Biology
>Department of biological and environmental science
>PO Box  35
>University of Jyväskylä
>Jyväskylä FIN 40014
>Finland
>+358 (0)14 260 4183 (work)
>+358 (0)414740463 (mob)
>
>http://www.chalkie.org.uk
>dan at chalkie.org.uk
>white at cc.jyu.fi