[vtkusers] green and red not mixing to make yellow, in raycastmapper???

[Dr. Daniel James White PhD]

Hi,

I've been following the discussion on the VTK list about ray casting 
and blending,
I am a novice so please bear with me....

I think I am experiencing this problem with VTK,
attached is a screen capture from MayaVi (VTK python frontend)
where I have loaded 2 data sets of a 3D volume of the same living cell 
taken by confocal microscopy.
These are 2 different fluorescence channels, which I represent as green 
and red.
(They are really from 2 grayscale tiff stacks)
Using the VTK raycastmapper with the compositefunction I expected to 
see yellow where there is signal in both the red and green channels (we 
call this colocalisation). same happens with the MIPfunction

But as you can see in the attached jpg I either get red or green only.
In the blobs (matrix vesicles) around the edge of the cell there should 
be some yellow visible.
You can see there are some blobs with green outer and red inner.....

Is this the same problem that you guys are talking about?

cheers

Dan


>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: redsandgreenraycast.jpg
Type: image/jpeg
Size: 3570 bytes
Desc: not available
URL: <http://www.vtk.org/pipermail/vtkusers/attachments/20031015/2fcb1c7a/attachment.jpg>
-------------- next part --------------
>
Dr. Daniel James White BSc. (Hons.) PhD
Cell Biology
Department of biological and environmental science
PO Box  35
University of Jyv?skyl?
Jyv?skyl? FIN 40014
Finland
+358 (0)14 260 4183 (work)
+358 (0)414740463 (mob)

http://www.chalkie.org.uk
dan at chalkie.org.uk
white at cc.jyu.fi