hi,maybe this code can help you,these files are DICOM3.0(512*512*16bit*200) vtkImageReader *reader=vtkImageReader::New(); reader -> SetDataByteOrderToLittleEndian(); reader -> SetFilePrefix(".../data/dicom"); reader -> SetHeaderSize(3606); reader -> SetDataExtent(0,511,0,511,1,200); reader -> SetDataMask(0xffff);